Barry Alan Kesner, PhD


Instructor in Investigation
Lee Lab, Mass General Research Institute
Research Associate in Genetics
Harvard Medical School
Research Staff
Molecular Biology, Massachusetts General Hospital
PhD University of North Carolina, School of Medicine 2009
bioinformatics; molecular biology; tissue culture My professional experience is highly varied. I have experience in both bioinformatics and wet lab research. 
My bioinformatics experience includes:
* larger protein alignments and tree generation
* homology modeling
* protein treading
* molecular dynamic simulation and analysis
* Discrete Molecuar Dynamics (DMD) and Amber
* Contact map analysis
* normal mode analysis
* analysis of DNA open reading frames
* unique mass spectra filtering software
* Languages: C, C++, Java, Perl
* Software: Mathematics, R, xmgrace, Mr. Bayes
* OS expertise in Linux

My wet lab work includes extensive studying Filamin, Cystic Fibrosis Conductance Regulator and the Adeno-associated Virus (AAV). To study these I used
* molecular biology
* tissue culture
* virus purification
* transduction assays in airway epithelial cells, hek293, and mice
* Assays I am familiar with include:
immuno-precipitaion, pulldown, western blots, 
southern blots, PCR, QPCR, DNA tissue extraction,
flow cytometry, luciferase, immunohisto chemistry,
use of confocal microscopes.
Publications
bkesner@partners.org
6176433285

Simches Building
185 Cambridge Street
Boston, MA 02114