Barry Alan Kesner, PhD
Investigator, Other (M) Molecular Biology, Mass General Research Institute |
Research Associate in Genetics Harvard Medical School |
Research Interests
Research Narrative
My bioinformatics experience includes:
* larger protein alignments and tree generation
* homology modeling
* protein treading
* molecular dynamic simulation and analysis
* Discrete Molecuar Dynamics (DMD) and Amber
* Contact map analysis
* normal mode analysis
* analysis of DNA open reading frames
* unique mass spectra filtering software
* Languages: C, C++, Java, Perl
* Software: Mathematics, R, xmgrace, Mr. Bayes
* OS expertise in Linux
My wet lab work includes extensive studying Filamin, Cystic Fibrosis Conductance Regulator and the Adeno-associated Virus (AAV). To study these I used
* molecular biology
* tissue culture
* virus purification
* transduction assays in airway epithelial cells, hek293, and mice
* Assays I am familiar with include:
immuno-precipitaion, pulldown, western blots,
southern blots, PCR, QPCR, DNA tissue extraction,
flow cytometry, luciferase, immunohisto chemistry,
use of confocal microscopes.